Fig. 2.

Orientation of the actin cytoskeleton and focal adhesions on microfabricated substrates. (A and B) Immunofluorescence images of filamentous actin (A) and protein vinculin (B) in MDCK cell islands grown on anisotropic PDMS micropillars (stiffest direction, horizontal). (C) Immunofluorescence staining of filamentous actin (green) in cells grown on a glass coverslip microprinted with Cy3-fibronectin (red). (D) Immunofluorescence staining of vinculin on a fibronectin-patterned coverslip (patches oriented horizontally). (Scale bars: 10 μm.)