Fig. 4.

BRC_60–89 reduces the rate of ATP hydrolysis by RAD-51. (a and b) Time course of ATP hydrolysis by RAD-51 alone or in the presence of BRC_21–50, BRC_60–89, or BRC_scram peptides. (c and d) The same ATPase assays were performed with the BRC_Wt (20–89) and BRC_Mut (14) (missing seven conserved BRC repeat residues) peptides. Reactions were performed in the presence of φX174 ssDNA (a and c) or φX174 dsDNA (b and d), with 4 mM Mg²⁺. Peptides were present at an 8-fold molar excess over RAD-51 ([RAD-51] = 1 μM). Error bars represent SEM from three independent experiments.