Fig. 5.

Comparison of the present Sec4p·Sec2p·phosphate complex and the nucleotide-free Sec4p·Sec2p complex structures (29) (stereo). The Sec2p structures in the two complexes are superposed over residues 100–120. Sec4p in the nucleotide-free complex is colored purple, and its P-loop is colored red. Sec4p in the Sec4p·Sec2p·phosphate complex is colored as in Fig. 1b, and its P-loop is colored yellow. The black arrows indicate the structural reorganization in Sec4p upon nucleotide dissociation.