Table 2.

Binding affinity of IN to the LTR DNA and the LEDGF/p75-derived peptides: Fluorescence anisotropy studies

Peptide	Kd for IN binding, μM	Hill coefficient
LEDGF/p75 353–378	4.4 ± 0.2	4.4
LEDGF/p75 361–370	3.7 ± 0.2	3.4
LEDGF/p75 402–411	12 ± 0.6	4.5
FL′ DNA LTR	0.034 ± 0.001	2.1
FL′ DNA LTR + LEDGF 361–370	0.099 ± 0.003	2.2
FL′ DNA LTR + LEDGF 353–378	0.068 ± 0.003	2.4
FL′ DNA LTR + LEDGF 402–411	0.20 ± 0.02	2.7
FL′ DNA LTR + full-length LEDGF/p75	1.1 ± 0.1	1.5

Binding curves are shown in Fig. 1. FL′, fluorescein-labeled.