Fig. 2.
The Arabidopsis mtp11-1 mutant is hypersensitive to Mn, whereas an MTP11 overexpressor is Mn-hypertolerant. (A) Growth of wild-type (Left) and mtp11-1 (Right) seedlings on a half-strength MS plate supplemented with 1 mM MnSO4. (B Left) Effect of Mn on root elongation of wild type (filled circles) and mtp11-1 mutant (open circles). Each data point is a linear regression of root elongation of 30 seedlings measured at 4, 6, 8, 11, and 13 days after germination. Regression coefficients of all points are >0.95. (B Right) Effect of Mn on shoot fresh weight of 17-day-old wild-type (filled circles) and mtp11-1 mutant (open circles) seedlings grown on horizontal plates. Data are the means ± SEM of three plates (25 seedlings per line and plate). (C) Growth of Col 0 (Left) and 35S::AtMTP11 line 8 (Right) on a near-vertical half-strength MS plate supplemented with 1.5 mM MnSO4. (D Left) Effect of Mn on root elongation of wild type (filled circles) and 35S::AtMTP11 line 3 (open circles). Experimental details are as in B. (D Right) Effect of Mn on shoot fresh weight of 13-day-old wild type (filled circles) and 35S::AtMTP11 line 3 (open circles) grown on near-vertical plates. Data are the means ± SEM of three plates (10 seedlings per line and plate). For A–D, experiments were repeated twice with similar results.