Table 1.
Leading ends |
Lagging ends |
||||||
---|---|---|---|---|---|---|---|
Wild type | tua5D251N | tua4S178Δ | Wild type | tua5D251N | tua4S178Δ | ||
Growth rate, μm/min | 4.68 | 2.80* | 7.46* | 1.48 | 1.56 | 2.31* | |
Shrinkage rate, μm/min | 11.53 | 6.90* | 11.91 | 5.50 | 1.49* | 6.36 | |
Catastrophe frequency, events/sec | 0.086 | 0.083 | 0.092 | 0.174 | 0.189 | 0.203 | |
Rescue frequency, events/sec | 0.152 | 0.177 | 0.143 | 0.145 | 0.146 | 0.107 | |
Time spent, growth | 76.8% | 63.4% | 52.2% | 9.1% | 14.7% | 8.4% | |
Pause | 9.1% | 15.4% | 9.8% | 46.2% | 55.4% | 56.9% | |
Shrinkage | 14.0% | 21.2% | 38.0% | 44.7% | 30.0% | 34.7% | |
Dynamicity, μm/min | 4.89 | 2.65* | 8.41* | 1.89 | 0.45* | 1.56 |
Wild type, n = 20 and t = 2,444 sec; tua5D251N, n = 10 and t = 1,260 sec; tua4S178Δ, n = 10 and t = 1,228 sec.
*Statistically significant difference from wild type (P < 0.05).