Table 4.
Fine mapping of WT1(286-293) as the minimal essential amino acid sequence using peptide-pulsed, autologous B-LCLs as APCs
| Peptide position | Sequence | Recognition (5% threshold) |
|---|---|---|
| 277-291 | TTPILCGAQYRIHTH | − |
| 281-295 | LCGAQYRIHTHGVFR | + |
| 285-299 | QYRIHTHGVFRGIQD | + |
| 286-300 | YRIHTHGVFRGIQDV | + |
| 289-303 | HTHGVFRGIQDVRRV | − |
| 284-294 | AQYRIHTHGVFR | + |
| 284-293 | AQYRIHTHGVF | + |
| 284-292 | AQYRIHTHGV | − |
| 285-294 | QYRIHTHGVFR | + |
| 285-293 | QYRIHTHGVF | + |
| 285-292 | QYRIHTHGV | − |
| 286-294 | YRIHTHGVFR | + |
| 286-293 | YRIHTHGVF | + |
| 287-294 | RIHTHGVFR | − |
T cells generated by stimulation with the 15-mer for WT-1(281-295) were tested by CFC for recogniton of autologous B-LCLs pulsed with the indicated peptides (10 μg/mL).