Skip to main content
. 2007 Mar 27;110(1):360–369. doi: 10.1182/blood-2006-12-065615

Figure 5.

Figure 5

R18 disrupts interaction between 14–3-3 and FOXO3a but not 14–3-3–BAD association in Ba/F3 cells transformed by ZNF198-FGFR1. (A) 4ZF-transformed TonBaF cells inducibly expressing YFP-tagged R18 dimer or mutant were cultured with Dox in the absence of IL-3 for 24 and 48 hours. Coimmunoprecipitated FOXO3a, BAD, and YFP-tagged R18 dimer or mutant in 14–3-3 immunoprecipitates were detected by specific antibodies. (B) Phosphorylation levels of FOXO3a, BAD, PI3K, AKT, and ERK in the cell lysates were probed by Western blotting as controls.