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Design of hypercleavable fragments of cI. (a) Crystal structure of the CTD dimer (PDB code 1F39)13 is shown with one monomer in cyan. Side-chains of active site residues are in orange, residues affected by Inds mutations are in magenta, and the C-terminal 310 helix, residues 230-236, in blue, (b) Cartoons representing full length cI (top) and the hyper-cleavable fragments used in this study (bottom) are shown with the residues of interest color coded as in (a) and Figure 4.
