Figure 1.
Schematic of the tissue sectioning regime in relation to cardiac coordinates X1, X2, X3. A block of anterior LV freewall myocardium is cut from the heart, and divided into two smaller blocks a and b. The block originates from the region of watershed between the left anterior descending (LAD) and circumflex (Cx) artery supply territories. Sections are taken from block a in the epicardial (X1-X2) plane, and from block b in both the base-apex (X2-X3) and circumferential (X1-X3) planes. Registration of sections from block b is aided by placement of fiducial rods prior to sectioning.