Figure 4.
Effect of SAP on immediate killing of E. coli J5 in SAP−/− mice in vivo. E. coli J5 was preincubated with whole acute phase serum from wild-type SAP+/+ mice, or from SAP−/− animals, or with SAP−/− acute phase serum reconstituted by the addition of isolated mouse SAP, and 107 organisms were then injected i.p. into individual C57BL/6 SAP−/− mice. After 15 min the peritoneal cavity was lavaged, and the numbers of bacteria surviving in each animal were determined. Horizontal lines indicate medians, and the P value (Wilcoxon rank sum test) indicates the significant difference between bacterial killing in the presence or absence of SAP.