Table 2.

Effect of human SAP binding on phagocytosis of bacteria by human neutrophils

Bacterium	Medium	Relative GFP or FITC fluorescence of ethidium positive neutrophils Mean (SD), n = 3
E. coli J5	TCB buffer	100.0 (10.3)
	SAP in TCB buffer	22.5 (4.6)
E. coli J5	Whole serum	32.9 (4.2)
	CRP-depleted serum	33.1 (0.9)
	SAP-depleted serum	100.0 (9.4)
	SAP-repleted serum	44.5 (7.1)
E. coli O111:B4	TCB buffer	100.0 (4.6)
	SAP in TCB buffer	97.7 (3.3)
E. coli O111:B4	Whole serum	94.4 (8.6)
	CRP-depleted serum	98.4 (18.0)
	SAP-depleted serum	100.0 (16.2)
	SAP-repleted serum	105.2 (17.2)
N. meningitidis H44/76	Whole serum	40.3 (7.7)
	SAP-depleted serum	100.0 (6.0)
N. meningitidis H44/76	SAP-depleted serum	100.0 (10.4)
	+ SAP 0.1 mg/l	91.9 (19.0)
	+ SAP 1.0 mg/l	89.3 (16.0)
	+ SAP 10.0 mg/l	62.6 (12.2)
	+ SAP 100 mg/l	47.3 (6.5)
	+ SAP 1000 mg/l	41.7 (7.4)