FIGURE 5.

Gene transfections with natural phospholipids and Ca²⁺ ions. (A) Relative transfection efficiency with varying concentrations of Ca²⁺ on U87 cells: egg PE (black ▪), egg PA (gray •), egg PC (◊). L/D = 7 and (B) Transfection efficiency of egg PE in relative light units per mg of proteins versus growth time of lipoplexes before transfection τ_g (bars, left axis), and their corresponding size (♦, right axis). L/D = 7 and (C) Relative transfection efficiency of egg PE as a function of both L/D and at fixed