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Far-UV circular dichroism. (A) Wavelength scans of cTir WT and mutants. The data display two overall patterns, as discussed in the text. Traces for WT, Ser-434-Asp, Ser-463-Ala, Ser-463-Asp, Ser-434-Ala/Ser-463-Ala, and Ser-434-Ala/Ser-463-Asp are solid; Ser-434-Ala, Ser-434-Asp/Ser-463-Ala, and Ser-434-Asp/Ser-463-Asp are shaded. (B) Wavelength scans for full-length protein (solid line, WT; dashed line, Ser-434-Asp/Ser-463-Asp).
