TABLE 2.

Hydrodynamic parameters for cTir/FL-Tir derived from AUC experiments (interference data)

	Sedimentation coefficient (S)			Mass (kDa)			Hydrodynamic radius (Å)
	s1	s2	s3	M1	M2	M3	Rs
cTir WT (150 mM NaCl)	1.20 ± 0.01	—	—	19.6 ± 0.4	—	—	38.8 ± 0.2
cTir WT (50 mM NaCl)	1.78 ± 0.10	2.61 ± 0.14	4.22 ± 0.1	18.5 ± 0.5	39.7 ± 2.1	68.2 ± 2.2	—
cTir WT-P	1.47 ± 0.01	—	—	19.3 ± 0.4	—	—	35.4 ± 0.5
cTir 434A	1.48 ± 0.08	—	—	20.3 ± 0.4	—	—	37.4 ± 0.3
cTir 434D	1.52 ± 0.03	—	—	19.5 ± 0.4	—	—	35.0 ± 0.9
cTir 463A	1.48 ± 0.02	—	—	20.5 ± 0.5	—	—	37.0 ± 0.7
cTir 463D	1.49 ± 0.04	—	—	18.1 ± 0.4	—	—	33.8 ± 0.4
cTir 434A/463D	1.48 ± 0.01	—	—	20.5 ± 0.2	—	—	37.3 ± 0.4
cTir 434D/463A	1.50 ± 0.01	—	—	19.9 ± 0.2	—	—	36.2 ± 0.3
cTir 434A/463A	1.46 ± 0.02	—	—	17.7 ± 0.7	—	—	33.8 ± 0.8
cTir 434D/463D	1.47 ± 0.02	—	—	19.5 ± 0.5	—	—	34.7 ± 1.2
FL-Tir WT (150 mM NaCl)	2.65 ± 0.01	—	—	60.5 ± 2.1	—	—	57.0 ± 0.6
FL-Tir WT (50 mM NaCl)	2.67 ± 0.02			58.4 ± 2.6			54.3 ± 2.9
FL-Tir WT-P	2.68 ± 0.03	—	—	58.5 ± 2.5	—	—	56.2 ± 2.4
FL-Tir 434D/463D	2.65 ± 0.03	—	—	61.9 ± 1.4	—	—	62.7 ± 0.7