Skip to main content
. 2007 Apr 27;93(2):483–495. doi: 10.1529/biophysj.106.098517

FIGURE 3.

FIGURE 3

Spermine inhibition Ij-Vj relationships under three different [KCl] conditions. (A) Schematic diagram of the experimental design for the asymmetric [KCl] and [spermine] experiments performed on homotypic wild-type rat Cx40 gap junctions expressed in N2a cells. The normalized steady-state Ij-Vj curves for representative spermine concentrations are shown for the symmetric 140 mM KCl (B), asymmetric 140/70 mM cis/trans KCl (C), or asymmetric 70/140 cis/trans KCl (D) conditions. Each data point represents the average from three to five experiments (average of 4.6 ± 0.6 experiments for each [spermine] tested). The solid lines represent the model Ij-Vj curves using Eqs. 5 or 6 that account for the Vj-dependent gating plus spermine block of Cx40 gap junctions under the indicated conditions.