TABLE 3.
Asymmetric KCl Vj-dependent spermine equilibrium dissociation constants (
)
Effective equilibrium dissociation constant,  (mM)
|
||||||
|---|---|---|---|---|---|---|
| Vj (mV) | 25% trans[KCl] | 50% trans[KCl] | 75% trans[KCl] | 75% cis [KCl] | 50% cis [KCl] | 25% cis [KCl] |
| 4.3 ± 1.1 (r) | — | — | — | — | 30.95* ± 9.22 (0.96) | 4.35 ± 0.61 (0.95) |
| 8.9 ± 1.0 (r) | 4.50 ± 1.41 (0.91) | 6.01 ± 5.61 (0.84) | 21.3* ± 5.3 (0.95) | 9.09 ± 5.16 (0.94) | 20.02* ± 4.68 (0.96) | 5.91 ± 0.41 (0.99) |
| 13.6 ± 0.9 (r) | 2.53 ± 0.25 (0.96) | 3.61 ± 1.37 (0.89) | 6.37* ± 1.91 (0.91) | 10.3 ± 3.12 (0.97) | 13.63* ± 2.29 (0.96) | 6.77 ± 0.76 (0.98) |
| 18.3 ± 0.8 (r) | 1.68 ± 0.30 (0.92) | 1.82 ± 0.44 (0.90) | 2.87 ± 1.39 (0.88) | 8.13 ± 2.36 (0.96) | 9.01* ± 1.93 (0.94) | 6.80 ± 1.89 (0.96) |
| 23.1 ± 0.7 (r) | 0.599 ± 0.163 (0.85) | 0.633 ± 0.109 (0.89) | 1.24 ± 0.33 (0.89) | 3.86 ± 0.58 (0.96) | 7.13 ± 2.78 (0.95) | 4.62 ± 0.87 (0.95) |
| 28.1 ± 0.7 (r) | 0.211 ± 0.075 (0.80) | 0.233 ± 0.055 (0.84) | 0.603 ± 0.112 (0.90) | 1.73 ± 0.43 (0.89) | 2.96 ± 0.91 (0.91) | 2.95 ± 0.51 (0.93) |
| 33.1 ± 0.6 (r) | 0.086 ± 0.023 (0.83) | 0.049 ± 0.033 (0.73) | 0.327 ± 0.049 (0.89) | 0.664 ± 0.150 (0.87) | 1.42 ± 0.28 (0.89) | 2.22 ± 0.36 (0.91) |
| 38.1 ± 0.5 (r) | 0.076 ± 0.012 (0.87) | 0.017 ± 0.008 (0.84) | 0.188 ± 0.040 (0.83) | 0.394 ± 0.092 (0.84) | 0.674 ± 0.135 (0.88) | 1.43 ± 0.34 (0.83) |
| 43.2 ± 0.5 (r) | 0.091 ± 0.020 (0.77) | 0.007 ± 0.005 (0.84) | 0.138 ± 0.012 (0.94) | 0.149 ± 0.045 (0.85) | 0.462 ± 0.087 (0.86) | 1.16 ± 0.26 (0.82) |
| 48.2 ± 0.4 (r) | 0.070 ± 0.018 (0.83) | 0.008 ± 0.002 (0.94) | 0.163 ± 0.043 (0.81) | 0.067 ± 0.039 (0.82) | 0.384 ± 0.069 (0.87) | 0.831 ± 0.291 (0.69) |
*
The Hill coefficient for the indicated dose-response curves was fixed to the mean value of 0.7.