TABLE 2.

Antibody tagging profile of the P2X receptors

Subunit expression	Number of particles bound to receptor	Receptor alone	%	Receptor plus anti-His6 Ab	%	Receptors plus anti-HA Ab	%
P2X2-His6 > P2X6-HA	0	311	99.4	692	58.4	513	74.3
	1	2	0.6	428	36.1	171	24.7
	2	0	0.0	64	5.4	7	1.0
	3	0	0.0	1	0.1	0	0.0
P2X2-HA > P2X6-His6	0	337	98.8	322	65.5	712	67.8
	1	3	0.9	164	33.3	287	27.4
	2	1	0.3	6	1.2	48	4.6
	3	0	0.0	0	0.0	2	0.2
P2X2-His6 < P2X6-HA	0	412	98.8	552	72.3	654	68.8
	1	5	1.2	198	25.9	249	26.2
	2	0	0.0	13	1.7	47	4.9
	3	0	0.0	1	0.1	1	0.1
P2X2-His6	0	-	-	-	-	629	99.1
	1	-	-	-	-	6	0.9
	2	-	-	-	-	0	0.0
	3	-	-	-	-	0	0.0