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. 2007 May 29;404(Pt 3):439–448. doi: 10.1042/BJ20061815

Figure 3. Protein expression and substrate specificity of PMT-1.

Figure 3

(A) SDS/PAGE analysis. Samples were stained for total protein using Coomassie Blue. Arrows correspond to the indicated molecular mass markers. Lane 1 shows the sonicated material (50 μg of protein) and lane 2 shows the final size-exclusion purified protein (2 μg of protein). (B) TLC analysis of the PMT-1 reaction products. PMT-1 (5 μg) was incubated for 30 min with phosphoethanolamine (lane 1), P-MME (lane 2) or P-DME (lane 3) and the products were isolated as described in the Experimental section. Positions of authentic standards for P-MME, P-DME and phosphocholine (PC) are indicated on the right.