Table 3. Summary of pmt-1 feeding-RNAi and chemical rescue phenotypes in C. elegans.
C. elegans at the L1, dauer or L4 stage were placed on NGM-agar plates containing the indicated compound (EA, ethanolamine; MME, monomethylethanolamine; DME, dimethylethanolamine), as described in the Experimental section. The observed phenotypes are indicated.
| Starting worm (P0) | Added compound | Phenotype |
|---|---|---|
| L1 | None | P0 sterility |
| 5, 10 or 30 mM EA | P0 sterility | |
| 5 or 10 mM MME | Fertile adults | |
| 5 or 10 mM DME | Fertile adults | |
| 30 mM Choline | Fertile adults | |
| Dauer | None | P0 sterility |
| 5, 10 or 30 mM EA | P0 sterility | |
| 5 or 10 mM MME | Fertile adults | |
| 5 or 10 mM DME | Fertile adults | |
| 30 mM Choline | Fertile adults | |
| L4 | None | F1 L1/L2 or L3 arrest; lethality |
| 5, 10 or 30 mM EA | F1 L1/L2 or L3 arrest; lethality | |
| 5 or 10 mM MME | Wild-type F1 | |
| 5 or 10 mM DME | Wild-type F1 | |
| 30 mM Choline | Wild-type F1 |