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. 2007 May 29;404(Pt 3):449–458. doi: 10.1042/BJ20070003

Figure 4. Effect of isoform-specific inhibitors on insulin signalling in 3T3-L1 adipocytes.

Figure 4

Overnight-starved 3T3-L1 adipocytes were incubated for 5 min with the indicated PI3K inhibitors or DMSO and stimulated or not with insulin (100 nM, 10 min). Whole-cell lysates were then analysed by Western blotting using specific antibodies. (A) Effect of p110α-specific inhibitor (PIK-75, 100 nM) and p110β-specific inhibitor (TGX-221, 100 nM) on the insulin-induced stimulation of PKB phosphorylation. A representative Western blot is shown in each of the upper panels and quantification is presented below. The relative stimulation of insulin over the basal level was taken as 100% and all values are calculated as a percentage of this. The results are means±S.E.M. for three independent experiments each performed in triplicate. (B) Dose–response effect of two other structurally unrelated p110α-selective inhibitors (PI-103 and SN 30693) on the insulin-induced stimulation of PKB phosphorylation.