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. 2007 May 29;404(Pt 3):459–466. doi: 10.1042/BJ20061611

Figure 4. CBP and BRG1 synergistically increase transactivation activity of the Neh5-related regions of CNC transcription factors.

Figure 4

(A) Activity of GBD–30aa fusion proteins. A diagram of the GBD–30aa fusion proteins is shown in the left-hand panel. The numbers shown represent the position in each mouse protein sequence. SW480 cells were co-transfected with 20 ng of Gal4–TATA–Luc reporter and 100 ng of each GBD–30aa protein expression plasmid (right-hand panel). Transfections and luciferase assays were performed as described in Figure 3(B). **P<0.01 relative to the reporter construct alone (Control) (Dunnett's test). (B–D) SW13 cells were co-transfected with 20 ng of pCEP4-Gal4-TATA-Luc reporter and 100 ng of each GBD–30aa fusion proteins: GBD–Nrf2-30aa (Nrf2-30aa; B), GBD–p45-30aa (p45-30aa; C) or GBD–Nrf1-30aa (Nrf1-30aa; D) along with 800 ng of CBP and/or 200 ng of BRG1 expression plasmids. Transfections and luciferase assays were performed as described in Figure 3(B). *P<0.05, **P<0.01 relative to Nrf2 alone (Dunnett's test).