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. 2007 May 29;404(Pt 3):403–411. doi: 10.1042/BJ20061510

Figure 4. Localization of hQSOX1a-V5-KDEL in CHO-tPA cells to the ER.

Figure 4

(A) CHO-tPA cells stably expressing hQSOX1-V5-KDEL were fixed and stained with a mixture of anti-V5 antibody (left-hand panels) and anti- ERp57 antibody (right-hand panels). Secondary antibodies were conjugated to Alexa Fluor® 448 (green) or Alexa Fluor® 594 (red). Cell nuclei were stained with DAPI (4,6-diamidino-2-phenylindole). (B) CHO-tPA cells expressing hQSOX-V5-KDEL were radiolabelled for 10 min in the absence (lane 1) or presence (lanes 2–10) of varying concentrations of DTT. Cells were lysed and tPA immunoisolated from lysates with anti-tPA antibody. Immunoisolated proteins were separated by SDS/PAGE under reducing (upper panel) and non-reducing conditions (lower panel).