Figure 6. Effect of K201 on [³H]ryanodine binding.

The effect of K201 on the Ca²⁺ dependence of [³H]ryanodine binding to RyR2-wt (A) and the RyR2 mutant N4104K (B) was determined at 500 mM KCl, 5 nM [³H]ryanodine and various concentrations of Ca²⁺ in the presence (open circles) and absence (filled circles) of 50 μM K201. Results are normalized to the maximum binding (100%) in the absence of K201and are shown as means±S.E.M. from three separate experiments.