Full-length polypeptides were aligned using the BLOSUM62 scoring matrix (ClustalW MSA). The following accession numbers were used for mammalian subtype sequence [Homo sapiens (Hs): IP3R1, Q14643; IP3R2, Q14571; IP3R3, Q14573; Rattus norvegicus (Rn): IP3R1, P29994; IP3R2, P29995; IP3R3, Q63269]. The in silico predicted X. tropicalis IP3R1 displays 98% amino acid identity with the X. laevis IP3R1 isolated from an oocyte cDNA library ([3], D14400).