Figure 2.

Testing for adjuvant activity of cell-sized beads, foreign proteins, and primary cells. (A) Similar to Fig. 1A except that OVA beads were injected with GL261 cells (10⁶), 25 μM latex beads (cell size, 2 × 10⁶), or 10 μM of latex beads coated with FBS (2 × 10⁶; 10⁶ beads with FBS passively absorbed and the other 10⁶ beads with FBS covalently conjugated). GL261 cells cultured in serum-free media gave nearly identical results as cells cultured with FBS (not shown). (B) Similar to Fig. 1A except 2 μg OVA/BioMag beads was injected with or without 10⁵ C57BL/6 splenocytes. Syngeneic splenocytes also enhanced CTL responses to gp120 beads in BALB/c mice (not shown). E:T ratio, effector-to-target cell ratio.