Figure 3.

The adjuvant effect is not mediated by secreted factors and does not require continued protein synthesis. (A) 10⁶ GL261 cells were untreated (open squares), subjected to two rounds of freeze thawing (closed circles), or treated with 8 μM emetine (closed diamonds; leading to complete cell death overnight) and then injected into C57BL/6 with 5 μg OVA//BioMag; or OVA/BioMag beads without cells were injected (open triangles). CTL priming was evaluated as described in Fig. 1A. (B) Emetine completely inhibits de novo protein synthesis. A20 cells (see Fig. 5B) were either untreated or treated with 8 μM or 0.02% azide (control), and cultured with radio-labeled methionine and cystine. Trichloroacetic acid precipitation assay of ³⁵S-labeled cells was performed as described in Materials and Methods. E:T ratio, effector-to-target cell ratio.