Figure 4.

UV treatment leads to higher adjuvant activity. (A) BALB/c 3T3 cells were UV-irradiated for 5 min, incubated at 37°C for 5 h, and then subjected to two rounds of freeze thawing. The indicated number of cell equivalents were mixed with 0.5 μg gp120/latex beads and injected into BALB/c mice. CTL priming was evaluated as described in Fig. 1C. (B) Identical to A except that the 3T3 culture was covered with plastic lids during the UV treatment. No response was seen in mice injected with antigen beads without cells. E:T ratio, effector-to-target cell ratio.