Figure 6.

The cytosol contains adjuvant activity. (A) EL4 cells were disrupted by nitrogen cavitation and fractionated by differential centrifugation. Cytosolic and nuclear fractions from 5 × 10⁴ EL4 cells were mixed with 2 μg OVA/latex beads, injected into mice, and assayed as described in Fig. 1A. Fractionated GL261 cells and 3T3 cells (in BALB/c mice) gave similar results. (B) 3T3 cells were UV-irradiated as described in Fig. 4A and then were fractionated as in A. Cytosolic fraction in quantities equivalent to 10⁵ and 10⁴ initial 3T3 cells was mixed with 0.5 μg of gp120/latex beads and injected into mice. An assay similar to the one described in Fig. 1C was performed. (C) Identical to B except that 3T3 cells were covered with plastic lids during the UV treatment. E:T ratio, effector-to-target cell ratio.