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The adjuvant effect is systemic and long lasting: 106 emetine-treated GL261 cells were injected either 10 days (-10 days) before, 2 days (-2 days) before, at the same time, or 2 days after the injection of 5 μg BioMag/OVA beads. The OVA beads and emetine-treated cells were either injected into the same flank (ipsilateral) or the opposite flank (contralateral). CTL priming was evaluated 9 days after injections as described in Fig. 1A. E:T ratio, effector-to-target cell ratio.
