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. Author manuscript; available in PMC: 2007 Jun 25.
Published in final edited form as: FASEB J. 2006 Dec 8;21(2):586–595. doi: 10.1096/fj.06-6681com

Figure 5.

Figure 5

αERKO follicles exhibit ectopic expression of the Leydig cell specific gene Hsd17b3. A. Shown is a photograph of a representative ethidium bromide stained agarose gel (inverted) of semi-quantitative RT-PCR for Hsd17b3, Cyp11a1 and Actb transcripts in wild type (WT) and αERKO day 5 follicles following 24 h of treatment with either vehicle (V), an aromatase inhibitor (AI) or an ER-antagonist (ICI). B. Shown is quantitative data (average ± SEM) from real-time RT-PCR for Hsd17b3 expression from these same experiments. αERKO follicles clearly exhibit ectopic Hsd17b3 expression relative to wild type follicles and this phenotype cannot be reproduced in wild follicles following acute withdrawal of endogenous estradiol synthesis (via an AI) or direct repression of ER action (via ICI), indicating it is a fixed phenotype in αERKO follicles prior to culture. Bars that do not share a letter are significantly different (P < 0.05). The data shown is one of two independent experiments that yielded comparable results. Sample sizes were 8–9 follicles per genotype, per treatment, per experiment.