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Decrease in the intracellular cAMP levels in CD4+ T cells by Tat. Primary CD4+ T lymphocytes were pretreated with either IBMX or equivalent volumes of DMSO dilution buffer for 45 min and then seeded in wells coated with dilution buffer (BSA), Tat alone, or anti-CD3/CD28 ± Tat. Cell extracts were analyzed for the amount of intracellular cAMP after 4 h of culture. Data are expressed as means ± SD of four independent experiments performed in triplicate.
