Figure 4.
Expression of the GFP gene in human embryonic telencephalic cells cultivated in 10% FCS infected with Bac-CMV-GFP. 105 cells were infected with the vector at a moi of 10 and harvested 3 h, 48 h, and 96 h postinfection. Half of the wells were treated with 5 mM sodium butyrate after infection. Each value is the average value of two independent transductions ± SD. (a) Percentage of GFP+ cells as evaluated by flow cytometry. (b) Intensity of fluorescence expressed as the mean of fluorescence quantified by FACS analysis.