Figure 3.

Inhibition of rotavirus infectivity by integrin ligands. MA104 cells were incubated with either 0.5 μg/ml of vitronectin or 10 μg/ml of fibronectin, collagen type I, chondroitin sulfate, laminin, glycophorin A, or BSA for 60 min at 37°C, washed, and infected with rotaviruses as described in the legend for Fig. 1. Data are expressed as percentage of the virus infectivity obtained when the cells were preincubated with MEM as control. The average numbers of foci representing 100% infectivity were 128, 123, and 141 for RRV, nar3, and Wa, respectively. The bars represent the standard error of at least three independent experiments performed in duplicate.