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Dot blot analysis of OVA production by recombinant Salmonella. Strains ATCC14028, ATCC14028(pKKOVA), PhoPc, and PhoPc(pKKOVA) were grown in conditions described in Material and Methods. Serial dilutions of bacterial lysates were analyzed by dot blotting onto a nitrocellulose membrane. Dilutions of soluble OVA were blotted to generate a standard curve. The membrane was reacted with anti-OVA antibodies.
