Stability and multimer resolution of various miniF plasmids.
(A) Cells harbored miniF plasmid pXX704
(sopABC; ●), pXX765
(soj, spo0J-parS,
parS; ▴), pXX762 (soj,
spo0J-parS; ■), pXX766
(Δsoj,
Δspo0J-parS;
□), pXX764 (parS; Δ), and a miniF plasmid
pXX705 (ΔsopABC; ○).
(B) Purified plasmid DNA (100 ng) was separated on a
0.6% agarose gel and detected with a chemiluminescence reaction after
Southern hybridization. OC, open circular. Lanes: 1, pXX704
(sopABC); 2, pXX705
(ΔsopABC); 3, pXX762
(soj, spo0J-parS); 4, pXX764
(parS).