Figure 1.

Schematic representations of vectors and two-hybrid fusion constructs. Vectors are shown as circles within which filled arrows represent the β-lactamase (bla) and chloramphenicol acetyl transferase (cat) genes, and the lacZ-T18 and T25-lacZα ORFs. Open and solid boxes represent the pMB1 and p15A origins of replication, respectively. The lacUV5 promoters are shown as open arrowheads that indicate the direction of transcription. Restriction maps for the DNA fragments encoding the CTA1T18 and T25ARF6 hybrid proteins are shown above the vectors. Dashed lines show fragments inserted into vectors to make the clones: CTA1 ORF (open box) linked to the T18 ORF (solid arrow) in pCTA1T18 and derivatives; T25 ORF (solid box) linked to ARF6 ORF (hatched arrow) in pT25ARF6. Numbers above ORFs correspond to the residues of the native proteins included in the fusion polypeptides.