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Northern blot analysis of rRVs. BSR cells were infected with rRV or rRV-VSV-G (moi of 1), and total RNA was isolated 24 h later. RNA was separated by electrophoresis and transferred to a nylon membrane that was hybridized with a probe specific for RV-N (A), VSV-G (B), or a cDNA fragment spanning the entire RV-ψ and a portion of the RV-L gene (ψ + L) (C). G/L = RV G + L bicistronic RNA, L = RV L, G = glycoprotein, N = RV-N.
