Figure 5.
Particle production and infectious titers of rRV and rRV-VSV-G. BSR cells were infected with rRV (A) or rRV-VSV-G (B) at an moi of 50 and labeled with [35S]methionine for 24 h over 4–28, 28–52, or 52–78 h. Labeled virions were purified twice over 20% (vol/vol) sucrose and disrupted. Then the viral proteins were separated by SDS/PAGE. Gels were analyzed by PhosphorImaging, and viral proteins were quantified. The ratio of N protein to M protein (N/M) per virus was determined along with the ratios of N protein to G protein (N/G). The lane with the highest amount of N protein was determined for each virus and set to equal 100 (N ratio lane) and the other values were calculated in proportion to 100. The N ratios of rRV and rRV-VSV-G were compared and estimated in the particles row. A portion of the initial supernatant was used to determine the infectious titer at each time point (titer).