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Transport kinetics of cVSV-G. Infected cells were pulsed with [35S]methionine for 10 min and chased for 0, 30, 40, 50, 60, 90, or 120 min. Cell extracts were incubated with or without endo H for 4 h at 37°C and immunoprecipitated with an RV G endoplasmic tail-specific antibody. Proteins were separated by SDS/PAGE and analyzed by PhosphorImaging. Endo H + or − indicates with or without endo H.
