Figure 3.

Clonal expansion from single CD133⁺ cells. (a) Clonal expansion of neural stem/progenitor cells. Neurospheres can be derived from a single sorted CD133⁺ cell directly isolated from fetal brain or from a single CD133⁺ cell from sorted/expanded cultured neurospheres. (b) Differentiation capacity of clonally derived neurosphere cells. Progeny of single cell-derived neurospheres can be differentiated into neurons (β-tubulin III, green) and astrocytes (GFAP, red). Nuclei were counterstained with Hoechst 33342 (blue).