Table 1.
NS-IC activity in FBr and sorted populations
| Population* | % in brain | NS-IC | NS-IC range |
|---|---|---|---|
| Brain cell control (n = 8) | 100 | 1/731 | 1/139–1435 |
| CD133− sorted (n = 8) | 96 | 1/4,344 | 1/745–4,760 |
| CD133+ sorted (n = 8) | 3.6 | 1/31 | 1/6–1/74 |
| Brain cell control (n = 3) | 100 | 1/1,132 | 1/661–1,435 |
| 5E12− sorted (n = 3) | 97 | 1/12,791 | 1/1,259–35,672 |
| 5E12+ sorted (n = 3) | 2.5 | 1/286 | 1/79–392 |
| Brain cell control (n = 3) | 100 | 1/1,030 | 1/399–1,435 |
| CD133+ CD24−/lo (n = 3) | 1.1 | 1/23 | 1/15–34 |
| CD133+ CD24hi (n = 3) | 1.7 | 1/63 | 1/38–105 |
Limiting dilution assays on the above cell populations were performed and NS-IC frequencies were determined by linear regression analysis.
*
All sorted populations, except brain cell controls, were also gated for CD34− CD45−, which represent 98–99% of FBr.