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The CAFE assay. (A) Schematic diagram. Liquid food, topped with an oil layer to minimize evaporation, is introduced via a glass capillary held in place by a pipette tip. The pierced bottom of the inner chamber provides humidity. (B) Fly feeding from the capillary. To facilitate visualization, a red dye has been added to the medium and can be seen in the proboscis and abdomen of the fly.
