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. Author manuscript; available in PMC: 2007 Dec 20.
Published in final edited form as: J Immunol Methods. 2006 Oct 2;317(1-2):64–70. doi: 10.1016/j.jim.2006.09.003

Fig. 1.

Fig. 1

Determination of copy number of DR4 alleles. Genomic DNA from 54 conventionally typed samples was assayed by QPCR-SSPP for DRA and pan-DR4 in a multiplex TaqMan reaction to determine the quantity of DR4 per genomic equivalent. The cycle (CT) during which each sample reached an arbitrary threshold of fluorescence was determined, and the delta CT was calculated as CT DR4 – CT DRA. The mean delta CT for samples with either one or two copies of DR4 alleles is shown; the difference between these means is significant by a two-tailed, unpaired t test.