Table I.
Oligonucleotide sequences for high resolution HLA typing of DRB1*04 alleles.
| Oligo Name | Description | Sequence (5′ to 3′) | Final concentration (nM) |
|---|---|---|---|
| GDR1 | DRA forward primer | AGGCCGAGTTCTATCTGAATCCT | 225 |
| GDR2 | DRA reverse primer | CGCCAGACCGTCTCCTTCT | 225 |
| GDR3 | DRA probe | VIC-CATAAACTCGCCTGATTG-MGB | 50 |
| GDR4 | Pan DR4 forward primer | CGTTTCTTGGAGCAGGTTAAACA | 225 |
| GDR5 | Pan DR4 reverse primer | GCACGTACTCCTCTTGGTGATAGA | 225 |
| GDR6 | Pan DR4 probe | FAM-TCCGTCCCGTTGAAGA-MGB | 50 |
| DR4 subtyping: | |||
| GDR7 | DR4 subtyping forward primer | CGTTTCTTGGAGCAGGTTAAACA | 225 |
| GDR8 | DR4 subtyping reverse primer | CTCGCCGCTGCACTGTG | 225 |
| GDR9 | DRB codon 57-gat probe | FAM-CAGTACTCGGCATCAG-MGB | 50 |
| GDR10 | DRB codon 57-agc probe | VIC-ACTCGGCGCTAGG-MGB | 50 |
| GDR11 | DRB codon 71-aag probe | FAM-AGCAGAAGCGGGC-MGB | 50 |
| GDR12 | DRB codon 71-agg probe | VIC-TGGAGCAGAGGCG-MGB | 50 |
| GDR13 | DRB codon 71-gag probe | FAM-GAAGACGAGCGGGC-MGB | 50 |
| GDR14 | DRB codon 74-gcg probe | FAM-TGTCCACCGCGGC-MGB | 25 |
| GDR15 | DRB codon 74-gag probe | VIC-TGTCCACCTCGGC-MGB | 100 |
| GDR16 | DRB codon 86-ggt probe | FAM-AAGCTCTCACCAACC-MGB | 50 |
| GDR17 | DRB codon 86-gtg probe | VIC-AAGCTCTCCACAACC-MGB | 50 |