Table 2.
Amplicon characteristics of primers used for the annotation of the contig and the transcription profiling.
| Gene symbol or primer's name |
Forward primer (5'-3') Reverse primer (5'-3') |
Ta (°C) Length (bp) |
Accession number amplicons | % nucleic acid identity/amino acid identity/amino acid positivity with described sequences |
| ECHS1 | GCAAAGAATGGGAAAGAACAGCA GGCTCAAAAACCCGCCAGA |
65 646 |
EF215853 | OariEST (CD288818): 96/93/94 BtauECHS1 (DQ058603): 96/87/94 HsapECHS1 (NM_004092): 86/80/92 |
| PAOX | GCATCTGGACACCTTCTTTGA GTCCTCCCACACCACCTG |
65 274 |
EF215855 | OariEST (DY499183): 99/100/100 BtauPAOX (DQ058602): 95/98/100 HsapPAOX (NM_152911): 90/88/94 |
| MTG1 | CAGCTACCGCTATCACCGAGGA GCGAGGACTTGCCCACGTT |
60 154 |
EF215854 | BtauMTG1 (DQ058604): 92/75/85 |
| SPRN | GCGAGGGTGCGTGTGAGG CCTGAGGTCCACGCCCAGTA |
68 236 |
- | BtauSPRN (DQ058606): 95/92/93 HsapSPRN (NM_001012508): 79/76/80 |
| LOC619207 | GGCTGGTCAACGGCAGCA GGCTCTGTCCCCACGCAGT |
65 220 |
EF215856 | HsapLOC619207 (NT_017795): 88/73/84 |
| CYP2E1 | AAGAAATTGACAGGGTGATTGG AGGGAAGGAGGTCGATGAAT |
60 117 |
EF215857 | OariEST (EE790798): 98/100/100 BtauCYP2E1 (DQ058608): 98/97/100 HsapCYP2E1 (NM_000773): 91/86/94 |
| SYCE1 | GACAGCGGCAAGGAGCAGTT GCTTCACATCCTCCAGCTTTGC |
65 149 |
EF215858 | BtauSYCE1(NM_001038149): 100/100/100 HsapSYCE1 (NT_017795): 90/90/90 |
| OariBAC273H7 UP | GGGACCATCCTGCTGTGACG TCCACTGTCTGCGTCGTCCTC |
65 328 |
EI184560 | - |
| OariBAC265G4 RP | TGAGAGGTAAGAAGACCACCAAA TCAACCGCAGAACTATGAACC |
63 287 |
EI184566 | - |
| OariBAC161G10 RP | CAGCCTTGACGCACTCCTTT TTTAGAACTGGGCCACACAGC |
63 200 |
EI184563 | - |
| ACTB | CGCAGACAGGATGCAGAAAGA GCTGATCCACATCTGCTGGAA |
60 148 |
DQ386889 | - |
| UP | CGACGTTGTAAAACGACGGCCAG | 55 - |
- | - |
| RP | CACAGGAAACAGCTATGACCATGATTACG | 55 - |
- | - |
For amplicons containing both exon and intron sequences (ECHS1, MTG1 and SYCE1), sequence identity and positivity is shown for the exon parts of the amplicon.