FIGURE 7. Inhibition of Ski augments TGF-β-induced growth inhibition in vitro. Cells were transfected with Ski siRNA or control siRNA or not transfected 24 hours prior to treatment with or without TGF-β (5 ng/mL; time 0). Nontransfected cells and cells transfected with control siRNA demonstrated similar growth curves, with a 2.5- to 3-fold increase in cell number at 72 hours. Cells transfected with Ski siRNA, however, demonstrated only a 50% increase in cell number (*P < 0.005).