FIG. 3.

Eosin Y staining of chitosan in fungal cells. C. neoformans cells were grown for 24 h, S. cerevisiae cells were grown for 48 h, and all cells were then stained with eosin Y. Bright field and fluorescein isothiocyanate panels are shown for each strain. Strains or deletions are indicated at the top left of panels, and all cells were grown vegetatively except for those in the top right panel depicting sporulating S. cerevisiae. Arrows indicate cells that have taken up eosin Y, and these cells are dead, as confirmed by trypan blue staining (data not shown). All photos are the same magnification (×1,000).