FIG. 9.
India ink staining of capsule of KN99α and deletion strains. Strains were grown for 5 days on DME medium at 30°C. Strains or deletion mutants are indicated at top left of each frame. Cells were mixed with a 1:4 dilution of India ink-to-H2O solution. Capsule widths of more than 200 cells/strain were measured from the edge of the cell wall to the distal edge of the capsule, and average capsule width is indicated on the bottom right of each frame. A cap59Δ strain is included as a negative control. Scale bar = 20 μm.