FIG. 5.

GMT1 and GMT2 are not coordinately expressed. Expression of each gene is plotted normalized to its expression in a reference pool (indicated by the dotted line). Values shown are the median values from three separate experiments using independently prepared RNA samples and performed in duplicate. The RNA samples indicated were prepared from JEC21 cells, which were grown overnight in the following media: YPD, LIM (low Fe), LIM without BPDS (low Fe − BPDS), DMEM, DMEM plus CO₂, PBS, PBS plus fetal calf serum (FCS), Littman's medium with 1 μg/ml thiamine (high thiamine), Littman's medium with 0.01 μg/ml thiamine (low thiamine), YNB, YNB plus H₂O₂ (H₂O₂), or YNB plus NaNO₂ (NaNO₂). All cells were grown at 37°C except for YPD and YNB medium, which were grown at 30°C, and YNB medium with H₂O₂ or NaNO₂, which was grown at 25°C. All growth was in room air, except the DMEM-plus-CO₂ sample, which was grown in a 5% CO₂ atmosphere. See Materials and Methods for additional details of growth media, preparation of the reference pool, and analysis.